Fig. 3: Subcellular localization of P. fendleri DGATs and TAG lipases in High Oil (HO) N. benthamiana leaf pavement cells.

Confocal micrographs of transiently expressing GFP fusions of P. fendleri PfeDGAT1 (A), PfeDGAT2 (B), PfeTAGL1 (C), PfeOBL1 (D), and PfeSDP1 (E). Oil bodies were stained with 2 µg/ml Nile Blue A for 30 min. Scale bar = 10 µm. F-H, Normalized fluorescence intensity of GFP and Nile Blue A along the broken lines in panel A (F), panel C (G), and panel D (H). Each experiment was repeated thrice independently with similar results.