Fig. 4: Yeast two-hybrid assay and BiFC assay showing interaction of PfeDGATs with different P. fendleri prey genes. | Nature Communications

Fig. 4: Yeast two-hybrid assay and BiFC assay showing interaction of PfeDGATs with different P. fendleri prey genes.

From: Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils

Fig. 4

A Prototypic growth assay of yeast containing bait pBT3C-PfeDGAT1 with various preys pPR3N-PfeTAG lipases and pPR3N-PfeDGATs on nonselective (SD-LT, left) and selective (SD-LTHA, right) media. Positive control yeast consisted of bait pCCW-Alg5 (Alg5-Cub-LexA) and prey pAI-Alg5 (Alg5-HA-NubI) and negative control had empty plasmids bait pBT3C and prey pPR3N. B Quantitative beta-galactosidase assay showing the strength of interaction of same bait and prey plasmid pairs. C Prototypic growth assay of yeast containing bait pBT3C-STE-PfeDGAT2 with various preys pPR3N-PfeTAG lipases and pPR3N-PfeDGATs on nonselective (SD-LT, left) and selective (SD-LTHA, right) media. D Quantitative beta-galactosidase assay showing the strength of interaction of same bait and prey plasmid pairs. E Confocal images of WT Nicotiana benthamiana (i, ii, iii, vi, vii, viii) and HO Nicotiana benthamiana (iv, v, ix, x) leaves co-infiltrated with i. nYFPC-PfeDGAT1 and cYFPC-PfeDGAT1 ii. nYFPC-PfeDGAT1 and cYFPC-PfeDGAT2 iii. nYFPC-PfeDGAT1 and cYFPC-PfeTAGL1 iv. nYFPC-PfeDGAT1 and cYFPC-PfeOBL1 v. nYFPC-PfeDGAT1 and cYFPC-PfeSDP1 vi. nYFPC-PfeDGAT2 and cYFPC-PfeDGAT1 vii. nYFPC-PfeDGAT2 and cYFPC-PfeDGAT2 viii. nYFPC-PfeDGAT2 and cYFPC-PfeTAGL1 ix. nYFPC-PfeDGAT2 and cYFPC-PfeOBL1 x. nYFPC-PfeDGAT2 and cYFPC-PfeSDP1. Scale = 25 µm. Each experiment was repeated twice independently with similar results.

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