Fig. 7: Knockdown of TAG remodeling genes (PfeDGAT1, PfeDGAT2, and PfeTAGL1) in Physaria. | Nature Communications

Fig. 7: Knockdown of TAG remodeling genes (PfeDGAT1, PfeDGAT2, and PfeTAGL1) in Physaria.

From: Identification of triacylglycerol remodeling mechanism to synthesize unusual fatty acid containing oils

Fig. 7

A Total lipid content of T2 seeds of selected lines. Data represents mean ± SD (n = 3) of three biological replicates. Lines significantly different (p < 0.05) than Physraia_fen32 determined by Ordinary one-way ANOVA or with Dunnett’s multiple comparisons correction are marked with a black asterisk. B Fatty acid composition of T2 seeds of selected lines. Physaria_fen32 was used as wild-type control. Data represents mean ± SD (n = 3) of replicates. Lines significantly different (p < 0.05) than Physraia_fen32 determined by two-way ANOVA with Dunnett’s multiple comparisons correction are marked with a black asterisk. See Supplementary Figs. S8 and S9 for the data of all the knockdown lines for each gene. Relative expression of PfeDGAT1, PfeDGAT2, PfePDAT1, and PfeTAGL1 genes in selected PfeDGAT1 knockdown lines (C), PfeDGAT2 knockdown lines (D), PfeTAGL1 knockdown lines (E). The gene expression was quantified by qRT-PCR and normalized to 18S internal reference gene. Physaria_Fen 32 was used as calibrator. Data represent the mean ± SD of three biological replicates. A significant difference between control and knockdown lines was determined using a multiple t-test with the Holm-Sidak method and a confidence interval of 95% (*p ≤ 0.33, **p ≤ 0.002 and ***p ≤ 0.001). Source data underlying A–E and exact p values are provided in the source data file.

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