Fig. 3: Exercise modulates epigenetic regulation of Acvr1c and Bdnf during consolidation and reveals a specific permissive signature. | Nature Communications

Fig. 3: Exercise modulates epigenetic regulation of Acvr1c and Bdnf during consolidation and reveals a specific permissive signature.

From: Specific exercise patterns generate an epigenetic molecular memory window that drives long-term memory formation and identifies ACVR1C as a bidirectional regulator of memory in mice

Fig. 3

A Schematic. Created with Biorender.com. B, C Epigenetic regulation of Acvr1c during consolidation is modified by exercise. B Exercise does not impact H3K9Me3/H3K9Ac occupancy at the Acvr1c promoter following sedentary delay periods: One-way ANOVA, Group (F(5,47) = 4.41, P = 0.002), (0-0-0: n = 8, 0-0-2: n = 8, 14-0-0: n = 10, 14-7-0: n = 10, 14-7-2: n = 9, 14-14-0: n = 8). C Engagement in either minimal (2-day), (**P < 0.01) or extensive (14-day), (****P < 0.0001) exercise reduces repressive H3K27Me3 at the Acvr1c promoter. Notably, this reduction occurs throughout the sedentary delay periods assessed (7-day), (****P < 0.0001) and (14-day), (****P < 0.0001) and following reactivating exercise (***P < 0.001): (One-way ANOVA), Group (F(5,49) = 9.377, P < 0.0001), (0-0-0: n = 10, 0-0-2: n = 10, 14-0-0: n = 10, 14-7-0: n = 10, 14-7-2: n = 7, 14-14-0: n = 8). D, E Epigenetic regulation of Bdnf IV during consolidation is modified by exercise. D Exercise does not impact H3K9Me3/H3K9Ac occupancy at the Bdnf IV promoter: One-way ANOVA, Group (F(5,51) = 1.44, P = 0.22), (0-0-0: n = 10, 0-0-2: n = 10, 14-0-0: n = 10, 14-7-0: n = 10, 14-7-2: n = 9, 14-14-0: n = 8). E Remarkably, engagement in either minimal (2-day), (****P < 0.0001) or extensive (14-day) (****P < 0.0001) exercise also reduces repressive H3K27me3 at the Bdnf IV promoter. Notably, this reduction also continues at the sedentary delay periods assessed (7-day), (****P < 0.0001) and (14-day), (****P < 0.0001) sedentary periods and following reactivating exercise (****P < 0.0001): (One-way ANOVA), Group (F(5,53) = 13.90, P < 0.0001), (0-0-0: n = 10, 0-0-2: n = 10, 14-0-0: n = 10, 14-7-0: n = 10, 14-7-2: n = 9, 14-14-0: n = 10). Results provide evidence for the ability of exercise to effectively remove repressive marks at two distinct promoters of genes critical for memory in a specific manner during consolidation. Tukey’s post hoc test: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, + P = 0.05. Data are presented as mean ± SEM. Source data are provided as a Source Data file.

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