Fig. 5: Applications of zCRISPR-Cas12a for multiplex genome editing.
From: Harnessing noncanonical crRNA for highly efficient genome editing
Analysis of the multiplex genome disruption efficiency in U2OS and HEK293T cells. Five matched sites (MSs) used in previous experiments (Fig. 3c) and other three matched sites57, COL8A1 (MS-C), FGF18 (MS-F), and P2RX5-TAX1BP3 (MS-P), were selected for multiplex genome editing assessed by NGS. Double, quadruple, sextuple, or octuple A-crRNAs, Z-crRNAs, or sgRNAs were used to form the corresponding RNPs followed by nucleofection into cells simultaneously. 2×: double-gene knockout; 4×: quadruple-gene knockout; 6×: sextuple-gene knockout; 8×: octuple-gene. Error bars, s.e.m.; n = 3.
