Fig. 3: Absence of the CHD results in deficient HPK1 kinase activity. | Nature Communications

Fig. 3: Absence of the CHD results in deficient HPK1 kinase activity.

From: HPK1 citron homology domain regulates phosphorylation of SLP76 and modulates kinase domain interaction dynamics

Fig. 3

A Western blot showing protein levels of Jurkat cells expressing the indicated form of HPK1. B Phosphorylation of SLP76 over a 30-min timecourse after TCR stimulation in Jurkat cells expressing the indicated version of HPK1. C Percent of SLP76 phosphorylation over a one-hour timecourse in Jurkat cells expressing various forms of HPK1. Data shown in (A–C) are for one representative experiment of three independent experiments (n = 3) with technical triplicates and means ± S.D. displayed in (C). Statistical significance was determined by two-way ANOVA with Tukey’s multiple comparisons test. p = 0.0485 for comparison between Control GFP+ and FL WT, p < 0.0001 for FL WT vs ΔCHD. n.s.= not significant. D Protein levels of either the full-length or ΔCHD form of all members of the MAP4K family of kinases as indicated. Data in (D) is from a single transfection in HPK1−/− Jurkat cells. Source data are provided as a source data file.

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