Fig. 2: Overexpression of LncDACH1 inhibits proliferation, migration, and phenotypic switching during VSMC dedifferentiation. | Nature Communications

Fig. 2: Overexpression of LncDACH1 inhibits proliferation, migration, and phenotypic switching during VSMC dedifferentiation.

From: LncRNA-LncDACH1 mediated phenotypic switching of smooth muscle cells during neointimal hyperplasia in male arteriovenous fistulas

Fig. 2

a The transfection efficiency of pcDNA3.0 LncDACH1 in VSMC was measured by qRT-PCR (n = 7). b The effect of overexpression of LncDACH1 on the proliferation capacity of VSMC was examined by CCK-8 assay (n = 3). c, d Effect of overexpression of LncDACH1 on the proliferation capacity of VSMC was examined by EdU staining assay (n = 3). Scale bar, 25 μm. e Effect on VSMC migration capacity after overexpression of LncDACH1 was examined by Wound Healing assay (n = 3). Scale bar, 100 μm. f Effect on VSMC migration capacity after overexpression of LncDACH1 was examined by Transwell assay (n = 3). Scale bar, 50 μm. g–k Effect of overexpression of LncDACH1 on VSMC differentiation phenotype markers and dedifferentiation phenotype marker protein levels by Western Blot assay (n = 5). PDGF-BB (P), control (CTRL), negative control (NC), overexpression (OE). The n numbers represent biologically independent samples. Data are presented as mean values ± SD (a, b, d, e, f, h–k). P-values were determined by two-sided nonparametric tests (a) and two-sided one-way ANOVA (b, d–f and h–k) by Bonferroni’s multiple comparisons test. Source data are provided as a Source Data file.

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