Fig. 3: Silencing LncDACH1 promotes VSMC proliferation, migration, and phenotypic switching during dedifferentiation. | Nature Communications

Fig. 3: Silencing LncDACH1 promotes VSMC proliferation, migration, and phenotypic switching during dedifferentiation.

From: LncRNA-LncDACH1 mediated phenotypic switching of smooth muscle cells during neointimal hyperplasia in male arteriovenous fistulas

Fig. 3

a Transfection efficiency of si-LncDACH1 in VSMC was examined using qRT-PCR (n = 5). b Effect of silencing LncDACH1 on the proliferation capacity of VSMC was examined by CCK-8 assay (n = 3). c, d Effect of silencing LncDACH1 on the proliferation capacity of VSMC was examined by EdU staining assay (n = 3). Scale bar, 25 μm. e Effect on VSMC migration capacity after silencing LncDACH1 was tested by Wound Healing assay. Scale bar, 100 μm (n = 3). f Effect on VSMC migration capacity after silencing LncDACH1 was examined by Transwell assay (n = 3). Scale bar, 50 μm. g–k Effect of silencing LncDACH1 on VSMC differentiation phenotype markers and dedifferentiation phenotype marker protein levels by Western Blot assay (n = 5). PDGF-BB (P), control (CTRL), negative control (NC), small interfering (SI). The n numbers represent biologically independent samples. Data are presented as mean values ± SD (a, b, d–f, h–k). P-values were determined by two-sided nonparametric tests (a) and two-sided one-way ANOVA (b, d–f and h–k) by Bonferroni’s multiple comparisons test. Source data are provided as a Source Data file.

Back to article page