Fig. 8: LncDACH1 mediates NIH in AVF mouse model.

a The diagram shows the construction strategy of smooth muscle cell-specific LncDACH1 conditional knockout mouse. b The diagram shows the construction strategy of adeno-associated Virus overexpressing LncDACH1 mouse. c, e HE staining of vascular tissue and morphometric analysis of the extent of vascular neointimal hyperplasia in mouse without established AVF in CTRL (n = 9) and CKO-LncDACH1 (n = 9), respectively. d, f HE staining of vascular tissue and morphometric analysis of the extent of vascular neointimal hyperplasia in mouse with established AVF in CTRL (n = 8) and CKO-LncDACH1 (n = 8), respectively. g, h The expression levels of SRPK1 and HSP90 proteins in vascular tissues of CTRL and CKO-LncDACH1 mouse with established AVF were examined using Western Blot assays (n = 5 at each group). i, k HE staining of vascular tissue and morphometric analysis of the extent of vascular neointimal hyperplasia in mouse without established AVF in AAV-Vector (n = 9) and AAV-LncDACH1 (n = 9), respectively. j, l HE staining of vascular tissue and morphometric analysis of the extent of vascular neointimal hyperplasia in mouse with established AVF in AAV-Vector (n = 9) and AAV-LncDACH1 (n = 8), respectively. m, n The expression levels of SRPK1 and HSP90 proteins in vascular tissues of AAV-Vector (n = 5) and AAV-LncDACH1 (n = 5) mouse with established AVF were examined using Western Blot assays. Arteriovenous fistula (AVF), conditional knockout (CKO), adeno-associated virus (AAV), control (CTRL). The n numbers represent biologically independent samples. Data are presented as mean values ± SD (e–h, k–n). P-values were determined by two-sided nonparametric tests (e–h, k–n). Source data are provided as a Source Data file.