Fig. 3: oHSV induces ZBP1-mediated PANoptosis in tumor cells.
From: Fn-OMV potentiates ZBP1-mediated PANoptosis triggered by oncolytic HSV-1 to fuel antitumor immunity
4T1 (a) and 4MOSC1 (b) tumor cells were infected with oHSV (MOI = 1) for 24 h, cellular protein was analyzed by western blotting to detect the effect of ZBP1 expression on PANoptosis-related executive proteins. Effect of ZBP1, GSDMD, GSDME, and MLKL expression on relative cell viability in the 4T1 (c) and 4MOSC1 (d) cells treated with oHSV (MOI = 1) for 24 h. Western blotting was done thrice independently with similar results. n.s. not significant. e Effect of ZBP1, GSDMD, GSDME, and MLKL expression on ATP (left) and LDH (right) release in 4T1 tumor cells after oHSV (MOI = 1, 24 h) treatment. n = 3 independent experiments. f Schematic illustration of injection of 4T1 tumor cells and the effect of ZBP1, GSDMD, GSDME, and MLKL expression on 4T1 tumor size after oHSV treatment. n = 5 mice. S.C. = Subcutaneous. g Schematic illustration of injection of 4T1 tumor cells and the effect of ZBP1, GSDMD, GSDME and MLKL expression on 4MOSC1 tumor size after oHSV treatment. n = 5 mice. Statistical significance was determined using two-tailed Student’s t test in (c–e) and two-way ANOVA with Tukey’s multiple comparisons test in (f, g). Data represent the mean ± s.e.m. Source data are provided in the Source Data file.
