Fig. 5: Western blot analysis of the NifD protein in WT (A, C) and ∆kaiA (B, D) cells grown under CL and incubated for nitrogen fixation assay in air (A, B) or argon (C, D). | Nature Communications

Fig. 5: Western blot analysis of the NifD protein in WT (A, C) and ∆kaiA (B, D) cells grown under CL and incubated for nitrogen fixation assay in air (A, B) or argon (C, D).

From: Endogenous clock-mediated regulation of intracellular oxygen dynamics is essential for diazotrophic growth of unicellular cyanobacteria

Fig. 5

Total cellular protein was probed with NifD antibody. Experiments were independently repeated three times with similar results. Cyanothece 51142 NifD protein was expressed in E.coli and purified protein was used as a positive control (E). Arrow showing 54 kD NifD specific band in the samples. The image is a superimposition of the 700 channel on the chemiluminescence channel recorded on a Li-Cor ImageQuant LAS-4000 imager. The Bio-Rad Precision Plus Dual Color Protein Standard was used as the molecular weight marker.

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