Fig. 1: Cryo-EM structure of dimeric human CRL3KLHL22.
From: A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3KLHL22
a Shown are the two steps of dimeric CRL3s assembly. The first step is the formation of a dimeric BTB core. The second step is the recruitment of two CUL3-RBX1 subunits by the BTB core and the formation of dimeric CRL3s. b SDS-PAGE gel of purified CRL3KLHL22. The result shown is representative of at least three biological replicates. Source data are provided as a Source Data file. c Domain schemes of CUL3, RBX1, and KLHL22. d, e Cryo-EM density map and cartoon model of dimeric CRL3KLHL22. The map was present with C2 symmetry with dimensions indicated in the top view (left); the side view (middle), and the bottom view (right), are also shown (d). The corresponding models of CUL3 (blue), RBX1 (red), and KLHL22 (yellow) in cartoon representation are shown in the bottom panel (e).
