Fig. 3: Fusogenicity of full-length RV3 F variants. | Nature Communications

Fig. 3: Fusogenicity of full-length RV3 F variants.

From: Universal paramyxovirus vaccine design by stabilizing regions involved in structural transformation of the fusion protein

Fig. 3

Cell-cell fusion assay using HEK293T cells transiently transfected with the F protein of the RV3 JS strain, or variants thereof, in a 15:1 ratio to HN carrying a H552Q substitution, to allow fusion activation, and mScarlet to mark the cytosol of transfected cells in red. Cell nuclei were visualized with Hoechst in blue. Syncytia can be recognized by the dilution of the mScarlet signal after fusion with non-transfected cells, and the clustering of nuclei. Scale bar represents 100 μm. Experiment was performed twice with similar outcome, data shown are from the same, representative experiment.

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