Fig. 5: Structure of stabilized RV3 preF. | Nature Communications

Fig. 5: Structure of stabilized RV3 preF.

From: Universal paramyxovirus vaccine design by stabilizing regions involved in structural transformation of the fusion protein

Fig. 5

A Structure of OnlyEcto2P. Two monomers are shown as surface representation, and one as cartoon. Different structural elements are color-coded and indicated. Boxes refer to stabilizing substitutions and refer to Fig. 5B−G. (HRA heptad repeat A (dark blue), HRC: heptad repeat C (purple), HB: helical-bundle (maroon), FP: fusion peptide (red), DI-DIII β-sheet (pink)) (B) P41 buried in the hydrophobic pocket forming hydrophobic contacts involving sequence distant residues. C Space filling double substitution Q89M + Q222I in its aliphatic pocket formed by three helices. D The N167P and L168P substitutions in the DI β-hairpin. E Turn stabilizing F335P substitution. F S470V on the threefold axis of the stalk. G S477V forming an unusual coiled-coil structure with neighboring W473 and L481 and a central water channel.

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