Fig. 7: TauSTED fluorescence microscopy of Parides arcas wing scales at ~50% development, showing the intimate association of chitin and actin in the developing ridge.
From: The actin cytoskeleton plays multiple roles in structural colour formation in butterfly wing scales
A, B Merge of actin (phalloidin, green) and chitin (CBD, magenta). Location of enlarged merged images (C, D) shown by dashed boxes. C, D Enlarged images showing a top-down (XY) view of the ridges with the larger actin bundles regularly spaced in between the splayed ridges. Coloured lines indicate the location of the orthogonal views; with the yellow line corresponding to the XZ orthogonal view (below) and the cyan line corresponding to the YZ orthogonal view (right). White pixels indicate colocalization between the actin and the chitin in the orthogonal views. Red line through the ridges in the XZ orthogonal view corresponds to the measured intensities (E, F) of actin and chitin by position (µm). Scale bar lengths: (A, B) 5 µm; (C, D) 2 µm.
