Fig. 8: Chemical perturbation of actin with cytochalasin D at 50% development.
From: The actin cytoskeleton plays multiple roles in structural colour formation in butterfly wing scales
Typical morphological phenotype of butterflies injected with cytochalasin D (A) and medium/DMSO (control, C) into the right forewing at 50% development and zooms of each (A → B, C → D) showing the discernible colour change of the iridescent region. E Reflectance spectra at the angle of maximum reflectance for control (red) and cyto-D treated (blue) wings. Shaded areas indicate standard error of the mean (for 21 cyto-D treated and 15 control individuals). F−M SEM imaging of typical cyto-d treated (F−I) and control (J, K) individual’s wing scales in the treated region at different magnifications. Differences in brightness of the ridges indicates differences in height. N Ridge number for cyto-d treated (n = 12) and control scales (n = 12). O Ridge curvature (κ) for cyto-d treated (n = 20) and control scales (n = 20). Black points indicate individual scales. Red points and lines indicate the mean and standard deviation respectively. Scale bars lengths: (F, G, J, K) 15μm, (H, L) 5 μm, (I, M) 1 μm.
