Fig. 2: Mutations at residue 813 influence the protease utilization during cell entry. | Nature Communications

Fig. 2: Mutations at residue 813 influence the protease utilization during cell entry.

From: Functional and antigenic characterization of SARS-CoV-2 spike fusion peptide by deep mutational scanning

Fig. 2

A Vero cell entry of VSVpps bearing various SARS-CoV-2 S constructs was measured by the relative light unit (RLU) in a luciferase assay. B Vero-TMPRSS2 cell entry of VSVpps bearing various SARS-CoV-2 S constructs. Each bar represents the mean of four biological replicates. Each data point represents one biological replicate. Deviations from the WT were analyzed by two-tailed t-tests. “ns” indicates no significance (i.e., p-value > 0.05). C, D The effects of C E64D (cathepsin inhibitor) or D camostat (TMPRSS2 inhibitor) on Vero cell entry of VSVpps bearing various SARS-CoV-2 S constructs are shown. Curves depicted in C are significantly different (p = 0.0088, two-way ANOVA). The mean and standard error of the mean (SEM) of four independent biological replicates are depicted.

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