Fig. 2: Adult human GPCs are transcriptionally and functionally distinct from fetal hGPCs. | Nature Communications

Fig. 2: Adult human GPCs are transcriptionally and functionally distinct from fetal hGPCs.

From: Repression of developmental transcription factor networks triggers aging-associated gene expression in human glial progenitor cells

Fig. 2

A Workflow of bulk RNA-Seq analysis of human adult and fetal GPCs. B Principal component analysis of all samples across three batches (fetal CD140a+, n = 12; fetal A2B5+/PSA-NCAM-, n = 3; adult A2B5+, n = 3; biologically independent samples). C Venn Diagram of Adult vs Fetal differentially expressed gene sets. (FDR < 0.01, Log2FC > 1, calculated with DESeq2). D IPA network of curated significant terms and genes (FDR < 0.001). Node size is proportionate to node degree. Color corresponds to enrichment in either adult (red) or fetal (blue) populations. E Bar plots of significant IPA terms by module. Z-Scores indicate predicted activation in fetal (blue) or adult (red) hGPCs. F Log2-fold changes and heatmap of network gene TPMs. Source data are provided as a Source Data file. Error bars ± SEM.

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