Fig. 3: Structural comparison of Na_VEh^WT and Na_VEh^ΔN.

a The superposition of Na_VEh^ΔN and Na_VEh^WT (gray) viewed from the extracellular side (Left) and from the intracellular side (Right). The distances between neighboring subunits are labeled. b Conformational changes of Na_VEh^ΔN (red) and Na_VEh^WT (gray). One subunit is shown in cartoon, and the other three are shown in half-transparent surface. The pore domains of two structures are superimposed. Red arrows indicate the conformational shifts. c The superposition of the VSDs of Na_VEh^ΔN (red) and Na_VEh^WT (gray). S1-S3 are shown as cylindrical helices and S4 is shown as cartoon. Gating charge residues are shown as sticks. d The activation gate of Na_VEh^ΔN (Left) and Na_VEh^WT (Right), respectively. The key hydrophobic residues are shown as sticks and spheres. e Selectivity filter comparison between Na_VEh^ΔN and Na_VEh^WT. On the right, conformational changes of the P2 helix between Na_VEh^ΔN and Na_VEh^WT. The red arrow indicates the conformational shift of P2 helix.