Fig. 6: Cell uptake of NPB(Cytc)*. | Nature Communications

Fig. 6: Cell uptake of NPB(Cytc)*.

From: Cell-penetrating protein-recognizing polymeric nanoparticles through dynamic covalent chemistry and double imprinting

Fig. 6

a–f Fluorescence imaging of MDA-MB-231 cells incubated 20 µM NPB(Cytc)*: a brightfield image of cells; b image of cells in the blue channel (to visualize the nucleus-binding Hoechst 33342); c image of cells in the red channel (to visualize NPB(Cytc)*); d overlay of the brightfield image and the red-channels image; e overlay of (b, c); f overlay of (a–c). g Cell viability in the MTT assay. h Percent uptake of NPB(Cytc)* by MDA-MB-231 cells as a function of the NP concentration. i Percent uptake of NPB(Cytc)* by MDA-MB-231 cells in the presence of different inhibitors: DOG+Azide 2-deoxy-d-glucose + sodium azide, MβCD methyl-β-cyclodextrin, Sucrose hypertonic sucrose, CPZ chlorpromazine. Data were presented as the mean ± SE, n = 3 independent biological samples.

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