Fig. 2: Resolution of lung inflammation is followed by a second wave of myeloid cell infiltration. | Nature Communications

Fig. 2: Resolution of lung inflammation is followed by a second wave of myeloid cell infiltration.

From: Post-resolution macrophages shape long-term tissue immunity and integrity in a mouse model of pneumococcal pneumonia

Fig. 2

WT C57BL6/J mice were administered intranasal S. pneumoniae with lungs digested for analysis by polychromatic flow cytometry. Using the gating strategy in panel (A) (FMOs are shown in blue to identify positive populations (red)) profiles of (B–E) monocytes, interstitial and alveolar macrophage population were identified with panels (F–H) providing further analysis in interstitial macrophage sub-types. Data were analysed by one-way analysis of variance (ANOVA) and Tukey’s multiple comparisons test. A p value of <0.05 was taken as the threshold of significance with graphical representation as; p < 0.05 = *, p < 0.01 = ** and p < 0.001 = *** and presented as mean ± SEM (n = 3–8 mice/group).

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