Fig. 5: Post-resolution prostaglandin synthesis regulates monocyte infiltration via EP4/CCL2. | Nature Communications

Fig. 5: Post-resolution prostaglandin synthesis regulates monocyte infiltration via EP4/CCL2.

From: Post-resolution macrophages shape long-term tissue immunity and integrity in a mouse model of pneumococcal pneumonia

Fig. 5

Whole lungs were isolated at the indicated times following intranasal S. pneumoniae for lipid extraction and analysis by LC-MS/MS revealing the profile of (A) PGE2 and (B) a representative reconstructed ion chromatogram. PCR analysis was carried out on (C, D) post-resolution macrophages to identify the source of PGE2 as well as (E, F) the predominant PGE2 receptor (EP) that is expressed on alveolar and interstitial macrophages, respectively, as well as post-resolution (G) T cell populations. A similar biphasic profile for prostacyclin (PGI2), measured as its stable derivative 6-keto PGF1α, is presented in (H, I) along with (J) the post-resolution cells that synthesise it. Data were analysed by one-way analysis of variance (ANOVA) and Tukey’s multiple comparisons test. A p value of <0.05 was taken as the threshold of significance with graphical representation as; p < 0.05 = *, p < 0.01 = ** and p < 0.001 = *** and presented as mean ± SEM(n = 3–5 mice/group).

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