Fig. 1: Maximum-likelihood phylogenetic tree of derived NosZ proteins, with nos operon arrangements in methanotrophic and non-methanotrophic bacterial strains.
From: Nitrous oxide respiration in acidophilic methanotrophs
The phylogenetic tree was constructed with IQ-TREE (IQ-TREE options: -B 1000 -m LG + F + R5) using aligned NosZ (details in Materials and Methods) and rooted at the mid-point. Bootstrap values ≥ 70% based on 1000 replications are indicated. The scale bar represents a 0.5 change per amino acid position. Organization of the nos operon in methanotrophic strains (labeled in blue text) and closely related non-methanotrophic bacteria are shown. The genes, represented by arrows, are drawn to scale. Homologs are depicted in identical colors. The NosZ amino acid sequences and gene arrangement information were retrieved using the following genome accessions: GCF_017310505.1, Methylacidiphilum caldifontis IT6; GCF_000010785.1, Hydrogenobacter thermophilus TK-6; GCF_011006175.1, Hydrogenobacter sp. T-8; GCF_900215655.1, Hydrogenobacter hydrogenophilus DSM 2913; GCF_000619805.1, Sulfurihydrogenibium subterraneum DSM 15120; GCF_000021565.1, Persephonella marina EX-H1; GCF_000022145.1, Anaeromyxobacter dehalogenans 2CP1; GCF_000013385.1, Anaeromyxobacter dehalogenans 2CP-C; GCF_003054705.1, Opitutus sp. ER46; GCF_000019965.1, Opitutus terrae PB90-1; GCF_901905185.1, Methylocella tundrae PC4; GCA_901905175.1, Methylocella tundrae PC1; CP139089.1, Methylocella tundrae T4; FO000002.1, Methylocystis sp. SC2; GCF_000025965.1, Aromatoleum aromaticum EbN1; GCF_022760775.1, ‘Candidatus Rhodoblastus alkanivorans’ PC3; GCF_000143145.1, Hyphomicrobium denitrificans ATCC 51888; GCF_000344805.1, Bradyrhizobium oligotrophicum S58; GCF_027923385.1, Methylocystis echinoides LMG27198; GCA_003963405.1, Methylocystis sp. AWTPI-1. * indicates that the nosZ genes are truncated due to genome fragmentation. Source Data contains genome annotation information for Methylocella tundrae T4, Methylacidiphilum caldifontis IT6, Methylocystis spp. (strains IM2, IM3, and IM4), and ‘Ca. Methylotropicum kingii’.
