Fig. 2: Aerobic and anaerobic growth of N2OR-containing and N2OR-lacking Methylocella and Methylacidiphilum strains on methanol.
From: Nitrous oxide respiration in acidophilic methanotrophs
Methylocella tundrae T4, Methylocella silvestris BL2, Methylacidiphilum caldifontis IT6, and Methylacidiphilum infernorum IT5 cells were grown in LSM medium supplemented with 30 mM methanol as the electron donor and NH4+ as the N-source. Aerobic growth of the 4 strains with O2 (A, D, G, J), anaerobic growth with N2O (B, E, H, K), and anaerobic growth without N2O (C, F, I, L) as the sole terminal electron acceptor were determined by optical density measurements at 600 nm, followed by measurements of O2 and N2O consumption in the headspaces of the culture bottles. Note that the trace O2 present at the start of the incubation in the anaerobic cultures without N2O did not contribute to obvious growth (C, F, I, L). All experiments were performed in triplicates. Data are presented as mean ± 1 standard deviation (SD), and the error bars are hidden when they are smaller than the width of the symbols. Source data are provided as Source Data file.
