Table 2 The effect of N2O addition on CH4-oxidizing cultures of Methylocella tundrae T4 growing in suboxic conditions

From: Nitrous oxide respiration in acidophilic methanotrophs

Culture condition

CH4 oxidized (mmol·L-1)

O2 reduced (mmol·L-1)

N2O reduced (mmol·L-1)

Increase in OD600

CH4 + O2

9.74 ± 0.39

14.93 ± 0.43

NA

0.114 ± 0.006

CH4 + O2 + N2O

12.19 ± 0.24

13.96 ± 0.41

10.15 ± 0.35

0.143 ± 0.002

  1. The experiment was performed in 2-liter sealed bottles (replicates) with 60 mL of LSM medium in an O2-limiting suboxic headspace with and without N2O (0.5% O2, 5% CH4, 5% CO2, and 0 or 1% N2O). Following the observation of N2O reduction in bottles containing N2O, the headspace O2 and N2O mixing ratios in the bottles were increased to approximately 1% and 2% (v/v), respectively. The reduction of N2O by the cultures increased CH4 oxidation and biomass compared to cultures containing only O2. Data are presented as mean ± 1 SD (n = 3). NA not available.
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