Table 1 Anisotropic data collection and structure determination

From: Structure of HIV-1 RRE stem-loop II identifies two conformational states of the high-affinity Rev binding site

 

SLII–tRNA

Data collection

  Wavelength (Å)

1.00003

  Space group

P21

  Molecules/AU

2

  Cell dimensions

   a, b, c (Å)

67.8, 81.3, 81.4

   α, β, γ (°)

90, 99.1, 90

  Resolution (Å)

57.2–2.85 (3.20–2.85)a

  No. of unique reflections

12,674 (635)

  I/σI

8.9 (1.0)

  Rmeas/Rp.i.m

0.10/0.05 (1.36/0.72)

  CC1/2

0.999 (0.487)

  Completeness spherical (%)

61.5 (11.8)

  Completeness ellipsoidal (%)

88.5 (68.3)

  Redundancy

4.0 (3.5)

Refinement

  Resolution (Å)

57.2–2.85 (3.13–2.85)

  Rwork/Rfree

0.22/0.25 (0.33/0.37)

  No. of atoms

   Nucleotide

5743

  R.M.S. deviations

   Bond length (Å)

0.003

   Bond angles (°)

0.735

  Mean B-factors (Å2)

   Nucleotides

121.13

  1. Single crystal was used for data collection.
  2. aValues in parentheses correspond to the highest resolution shell.
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