Fig. 1: FMNL1 is required for the actin wave.
From: An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis
A Timelapse images of interphase actin wave in HeLa cells; F-actin and mitochondria marked with Lifeact-GFP and mito-DsRed2, respectively. Yellow arrow and dotted line indicate the position of the wave. Insets are displayed with enhanced contrast for ease of viewing. Scale bar 10 μm for whole cell images, 2 μm for insets. B Representative images of phalloidin (F-actin marker) and anti-TOMM20 (mitochondrial marker) in interphase cells treated with siRNAs to either FMNL1, 2 or 3. Scale bar 10 μm for whole cell images and 2 μm for insets. Cell boundaries are outlined in cyan. C Quantitation of actin wave size for each group in interphase cells, where Whiskers represent 10–90th percentile, center lines indicate medians, and plus signs indicate means. n = 60 across 3 biological replicates. D Percent of cells displaying an actin wave, bars represent medians. n = 3 biologically independent samples. E Quantitation from western blot and qPCR experiments examining the knock-down efficiencies of FMNL1, 2 and 3, siRNAs. Bars represent means. n = 3 and 4 biologically independent experiments for western blot and qPCR experiments, respectively. F Representative images of GFP-FMNL1 recruitment to actin wave-positive mitochondria, where F-actin is marked by phalloidin and mitochondria are marked by a TOMM20 antibody. Whole cell image scale bar is 10 μm, medium zoom inset scale bar is 5 μm, and high zoom scale bar is 1 μm. Experiment repeated 3 times with similar results. Accompanying are line scans where all signal is normalized to cytosolic signal. A–F Differently colored points indicate different biological replicates. Statistical test used was Kruskal-Wallis test with Dunn’s multiple comparisons. Source data are provided as a Source Data file. *, **, and *** indicate p-values of < 0.05, ≤ 0.005, and ≤ 0.0005 respectively.
