Fig. 4: Mitochondrial polarization does not feedback to control the actin wave.
From: An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis
A Timelapse images of live interphase cells expressing Lifeact-miRFP 703 (actin marker) and GCaMP6s (cytosolic calcium indicator) and stained with MitoTracker Red CMXRos. B Distribution of cells with different actin/mitochondrial phenotypes in the absence or presence of 20 μM CCCP. Bars represent means with SEM. 3 independent experiments. C Timelapse images of live interphase cells expressing Lifeact-GFP, mito-sBFP2, and stained with TMRE (mitochondrial membrane potential indicator) for cells without CCCP addition or cells treated with 20 μM CCCP. Display scaling for the actin and mitochondrial channels was altered between groups for ease of viewing. D Wave speed for untreated and CCCP-treated cells. Means with SEMs are shown; statistical analysis performed using a two-sided Student’s T test was employed. n = 6 and 5 for untreated and post-CCCP conditions across 3 biological replicates, respectively. Note: different cells observed before and after CCCP addition. A–D Dotted cyan lines denote cell boundaries and yellow arrows indicate positions of actin waves. Scale bars are 10 μm. In graphs, different colors represent different biological replicates. Source data are provided as a Source Data file. *, **, and *** indicate p-values of < 0.05, ≤ 0.005, and ≤ 0.0005 respectively.
