Fig. 5: The interphase actin wave promotes mitochondrial hetero-fusion.

A Timelapse images of region of interest in live interphase cell expressing Lifeact-GFP (F-actin marker) and mito-DsRed2. Two distinct mitochondria are outlined in yellow and cyan and depicted as a cartoon. Scale bar is 2 μm. B Timelapse images of live interphase cells expressing mito-photoactivatable GFP and mito-DsRed2 after knock down of FMNL1. The total mitochondrial channels were individually scaled for ease of viewing. Approximate segmented PA-GFP signal is shown as a mask. Scale bar is 20 μm for images of whole cells, 10 μm for insets. Accompanying is quantitation for this experiment, where percent change in PA-GFP area was measured. Means and SEMs are depicted and a two-sided Student’s T test was performed. n = 21 cells across 7 independent experiments. C Quantitation from manual tracking of mitochondria recently sampled by actin wave and mitochondria not recently sampled by actin wave. Cells were expressing Lifeact-mScarlet and mito-mEmerald. Bars represent medians and a two-sided Mann-Whitney test was performed for statistical analysis. n = 11 cells across 3 independent experiments. D Cartoon depicting how the interphase actin wave might promote mitochondrial hetero-fusion. A–D In graphs, different colors represent different biological replicates. 3 or more biological replicates. Source data are provided as a Source Data file. *, **, and *** indicate p-values of < 0.05, ≤ 0.005, and ≤ 0.0005 respectively.