Fig. 4: Metabolic adjustments during growth on air.

Comparative proteomics of M. gorgona MG08, M. rosea SV97, and M. palsarum NE2 exposed to 1000 p.p.m.v. CH₄ (High) in air and 1.9 p.p.m.v. (Atm) CH₄ in air. Normalized and standardized expression of enzymes involved in the central carbon and energy metabolism is shown. High relative abundance = orange, low relative abundance expression = blue. * indicates significant difference in expression between treatments (two-sided t-test). Horizontal lines in the heatmaps separate operons of enzymes catalyzing the same reaction.? = unknown enzyme. Abbreviations: pMMO—particulate methane monooxygenase, MDH—methanol dehydrogenase (Mox) and (XoxF), Hhy— [NiFe] hydrogenase, CODH—carbon monoxide dehydrogenase, Fae—formaldehyde activating enzyme, MtdB—NAD(P)-dependent methylene-tetrahydromethanopterin dehydrogenase, Mch—methenyl-tetrahydromethanopterin cyclohydrolase, Fhc—formyltransferase/hydrolase, Fdh A—NAD-dependent formate-dehydrogenase, Fdh AB—molybdopterin dependent formate dehydrogenase, Fhs—formate-tetrahydrofolate ligase, FolD—bifunctional methylene-tetrahydrofolate dehydrogenase/methenyl-tetrahydrofolate cyclohydrolase, FchA—methenyl-tetrahydrofolate cyclohydrolase, MtdA—NADP-dependent methylene-tetrahydrofolate dehydrogenase, GcvH—glycine cleavage system H protein, GcvT—aminomethyltransferase, GcvP—glycine dehydrogenase, Lpd—dihydrolipoyl dehydrogenase, GlyA—serine hydroxymethyltransferase, SgaA—serine-glyoxylate aminotransferase, HprA—glycerate dehydrogenase, GarK – 2-glycerate kinase, Eno—enolase, Ppc—phosphoenolpyruvate carboxylase, Mdh—malate dehydrogenase, Mal—malate-CoA ligase, Mcl—L-malyl-CoA lyase, Acc—Acetyl-CoA carboxylase, NAD⁺—nicotinamide adenine dinucleotide, NADP⁺—nicotinamide adenine dinucleotide phosphate, ATP—adenosine triphosphate. Source data are provided in the Source Data file and in the Supplementary Data file (Dataset 5).