Fig. 2: Testing transduction of AAV8 and AAVv128 capsid following subretinal injections.
From: An adeno-associated virus variant enabling efficient ocular-directed gene delivery across species
a OCT images showing retinal blebbing formed at the site of subretinal administration. Representative animal eyes were shown at post-injection of necropsy (1 μL volume, n = 6). b Fluorescence fundoscopy of mouse eyes treated with PBS (1 μL volume, n = 6, scale bar: 500 μm). c–f Fluorescence fundoscopy of mouse eyes treated with ssAAV-CBA-eGFP vectors packaged with AAV8 (c) or AAVv128 (d) capsids (1×109 vg/eye, 1 μL volume, n = 6, scale bar: 500 μm). Mice were imaged at D14 and D28 post-injection, and each image of pixel intensity and mean pixel intensity per pixel area was quantified by using Image J (e, f). g, i Mice where sacrificed at D28 and eyes were harvested. Flat mounts of the retina were prepared and imaged by fluorescence microscopy to visualize native eGFP expression (g) and quantify by pixel intensity and mean pixel intensity per pixel area (i). Scale bar: 500 μm. h, j Mice retinal tissues were isolated and the relative eGFP copy number per cell were measured by qPCR. Values represent mean ± SD. P Values were determined by one-way ANOVA. Compared with PBS, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Compared with AAV8, #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001. n = 3/group (i). ns not significant. n = 6/group (e, f, j). Source data are provided as a Source Data file.
