Fig. 3: Upper SC-pH zone shows an acclimation-like property.
From: Three stepwise pH progressions in stratum corneum for homeostatic maintenance of the skin
a High-magnification X-Y plane representative confocal images of the dorsal skin of VmCSG1 mice soaked in STELLA Fluor 650. Merged images of Venus (green), mCherry (red), and STELLA Fluor 650 (blue) are shown. Scale bar, 1 μm. b High-magnification X-Y plane representative confocal images of the dorsal and abdominal skin of VmCSG1 mice treated with or without various pH buffers (pH 4.1, 5.3, 6.3, and 7.2). Scale bar, 5 μm. c pH in the upper SC-pH zones of dorsal or abdomen skin of VmCSG1 mice treated without buffer (no water) and various pH buffers (pH 4.1, 5.3, 6.3, and 7.2) (n = 22, 20, 20, 24, 25, 25, 30, 20, 20, and 22 spots from three biologically independent animals, respectively). d pH in the middle SC-pH zones of dorsal or abdomen skin of VmCSG1 mice treated without buffer (no water) and various pH buffers (pH 4.1, 5.3, 6.3, and 7.2) (n = 25, 20, 33, 27, 25, 25, 25, 25, 25, and 25 spots from three biologically independent animals, respectively). e pH in the lower SC-pH zones of dorsal or abdomen skin of VmCSG1 mice treated without buffer (no water) and various pH buffers (pH 4.1, 5.3, 6.3, and 7.2) (n = 25, 25, 25, 28, 29, 28, 23, 20, 21, and 25 spots from three biologically independent animals, respectively). Data are shown as mean ± SEM and are pooled from three experiments (c–e) or are representative of at least three independent experiments (a and b). *p < 0.05, ****p < 0.0001; Kruskal-Wallis one-way ANOVA followed by Dunn’s post hoc test (c–e). Source data are provided as a Source Data file.
