Fig. 7: TTM attenuated MI/RI and cuproptosis in mice with sleep fragmentation (SF). | Nature Communications

Fig. 7: TTM attenuated MI/RI and cuproptosis in mice with sleep fragmentation (SF).

From: Sleep fragmentation exacerbates myocardial ischemia‒reperfusion injury by promoting copper overload in cardiomyocytes

Fig. 7

A Schematic of the experimental protocol used to establish TTM-exacerbated MI/RI. Cartoon mouse images was drawn by Figdraw (https://www.figdraw.com/#/). B TTM reduced the copper ion level in the myocardium in SF mice before and after MI/RI, as detected by ICP‒MS (n = 5, 5, 6, 6 mice for DMSO, TTM, DMSO + I/R and TTM + I/R). The significance of differences was evaluated using an unpaired two-tailed multiple t-tests. *P < 0.05, **P < 0.01, ***P < 0.001. C Representative M-mode echocardiographic changes. D–F Statistical analysis of Left ventricular ejection fraction (LVEF), Left ventricular fractional shortening (LVFS) and Left ventricular internal diameter, systolic (LVIDs) (n = 10 mice per group). G Representative TTC staining of the myocardium. Scale bar, 2 mm. H The percentage of infarct area was determined by TTC staining (n = 5 mice per group). I, J Representative images of DLAT oligomers from both wide-field and confocal immunofluorescence images of the heart by confocal microscopy (red: DLAT oligomers, blue: DAPI), and the levels of DLAT oligomers quantified by calculating the mean fluorescence intensity (n = 10 mice per group). K Validation of the levels of DLAT oligomerization, lipoylated proteins, iron-sulfur cluster proteins and HSP70 by Western blotting. L–Q Statistical analysis of DLAT oligomers, DLAT monomer, Lip-DLAT, Lip-DLST, FDX1 and HSP70. Data on these proteins were normalized to GAPDH (n = 6 mice per group). Data are presented as the mean ± s.e.m. The significance of differences was evaluated using one-way ANOVA. Source data are provided as a Source Data file.

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