Fig. 6: Transient overexpression, silencing and functional analysis of CcOMT1. | Nature Communications

Fig. 6: Transient overexpression, silencing and functional analysis of CcOMT1.

From: An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis

Fig. 6

a Expression profiles of CcOMT1 after infiltration with CcOMT1-pCAMBIA1301. b Changes in 3,5,6,7,8,3’,4’-heptamethoxyflavone (HPMF) content after transient overexpression of CcOMT1 in CRC peel. The effect of transient expression of CcOMT1 on the content of HPMF in the peel of CRC was measured after infiltration with CcOMT1-PCAMBIA1301, with the empty vector as a control. Error bars, mean ± s.d, n = 4. The experiment was repeated twice. *** indicates a significant difference between the two groups according to a one-tailed Student’s t test. c Relative expression of CcOMT1 in virus-induced CcOMT1 silencing CRC fruits. d Changes in HPMF content in CRC fruits after virus-induced silencing of CcOMT1. CK, empty TRV2 vector control fruits; VIGS, virus-induced CcOMT1 gene silencing fruits. The content of flavonoids is expressed as mg·g-1 FW. Error bars, mean ± s.d, n = 8. The experiment was repeated twice. *** indicates a significant difference between the two groups according to a one-tailed Student’s t test.

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