Fig. 5: Distribution of apoptotic debris and DNASE1L3 in human and mouse.
From: Double-negative B cells and DNASE1L3 colocalise with microbiota in gut-associated lymphoid tissue
a Visualisation of apoptotic debris (TUNEL, red), cell nuclei (Iridium, blue), CD68 (green) and E-cadherin (orange) obtained using an IMC-TUNEL combined protocol in a section serial to that shown in Fig. 4a. b Distribution of CD11c (yellow) and CD163 (cyan) on same image as in (a). Subepithelial dome (SED) and germinal centre (GC) are highlighted (green and grey dashed lines respectively). c Magnification of regions in (a, b), showing individual and merged images for each marker. d RNAscope analysis showing the distribution of cell nuclei (DAPI, blue), DNASE1L3 transcripts (cyan, pseudocolor) and lysozyme protein (magenta, pseudocolor) in mouse GALT (Peyer’s patch) assessed through confocal microscopy, including demarcation of the SED (green dashed line) and the GC (grey dashed line). e Visualisation of apoptotic debris with TUNEL assay (green) and lysozyme protein expression (magenta, pseudocolor) through confocal microscopy in the SED (green dashed line) and the GC (grey dashed line) of mouse GALT. Scalebars = 100 μm. Findings for (a, b, c) are representatitve of 16 ROI. Findings shown in (d, e) are representiative of n = 5 C57BL/6 mice and n = 2 CBA mice.
