Fig. 7: Co-expression of C1Q with DNASE1L3 and C1R with C3.
From: Double-negative B cells and DNASE1L3 colocalise with microbiota in gut-associated lymphoid tissue
a RNAscope coupled with confocal microscopy showing cell nuclei (DAPI, blue), DNASE1L3 (green) and C1Qb (red) transcripts in the subepithelial dome (SED) (green dashed line) and germinal centre (GC) (grey dashed line) of an appendix follicle. b RNAscope coupled with confocal microscopy showing cell nuclei (DAPI, blue, pseudocolor), C1r (cyan, pseudocolor), C3 (magenta, pseudocolor) and C1Qb (red, pseudocolor) transcripts in an appendix follicle. c RNAscope coupled with confocal microscopy showing cell nuclei (DAPI, blue), Vimentin protein (yellow, pseudocolor), C3 (magenta, pseudocolor) and C1R (cyan, pseudocolor) transcripts in a human appendix follicle. Scalebars =100 μm. Findings shown in (a, b, c) are representiative of 6, 9 and 4 ROI respectively.
