Fig. 6: circCDK13OE-sEVs accelerate wound healing in db/db diabetic mice.
a Schematic diagram of skin wound, sEVs subcutaneous injection, and skin wound harvest timeline in db/db diabetic mice. b Representative images of the wound area by different treatments on days 0, 3, 7, 14, and 21 after operation. c Simulation plots of the wound closure areas. d Representative images of H&E staining of wound sections. Scale bar, 1 mm. e High-magnification images showed wound re-epithelialization, granulation tissue formation, and regeneration of skin appendages (hair follicles and sebaceous glands). Scale bar, 200 μm. f Representative images of Masson’s trichrome stating of wound sections. Scale bar, 200 μm. g Quantitative evaluation of the wound closure rate (n = 6 biologically independent samples). h Quantitative analysis of wound width (n = 6 biologically independent samples). i Quantitative analysis of epithelial tongue length (n = 6 biologically independent samples). j Western blot analysis of IGF2BP3, c-MYC, CD44, and cyclin D1 protein expression levels in skin wound tissue of different treatment groups. k Immunofluorescent staining of IGF2BP3, CD44, and c-MYC in epidermal keratinocytes of skin wounds. Scale bar, 50 μm. l Immunofluorescent staining of IGF2BP3, CD44, and c-MYC in dermal fibroblasts of skin wounds. Scale bar, 50 μm. DAPI staining was used to label the nuclei (blue). Vimentin staining was used to label HDFs (red). In (j, k, l) three independent experiments were performed and similar results were obtained. Comparisons were performed by one-way ANOVA followed by Tukey’s multiple comparisons test in (h, i). Data are presented as mean values ± SD. Source data are provided as a Source Data file.
