Fig. 6: Microdiversity (intra-population diversity; mean π) of Sargasso Sea viral populations.
From: Long-read powered viral metagenomics in the oligotrophic Sargasso Sea
(Calculated as Gregory et al. 30): A The microdiversity of Sargasso Sea viral populations from 200 m and 80 m samples (mean π: 3.119 × 10−4 (2.347 × 10−4 – 4.094 × 10−4, 95% CI) and 3.967 × 10−4 (3.072 × 10−4 – 4.909 × 10−4, 95% CI), respectively). On average, microdiversity was ~30% greater in viral genomes from 200 m than those from 80 m samples, but this was not a significant difference (permuted significance test: p = 0.164; Supplementary Fig. 10B). B VirION (long-read assembly and hybrid long- and short-read assembly) facilitated the capture of viral genomes with high microdiversity: mean π was 388.668% (264.559 – 551.95%, 95% CI) greater for viral genomes captured by VirION (n = 1465) compared to those assembled from short-reads (n = 2049) (permuted significance: p < 0.001; Supplementary Fig. 11B).
