Fig. 3: Cryo-ET 3D structures illustrating the dynamics of di-, tri- and tetranucleosome array particles. | Nature Communications

Fig. 3: Cryo-ET 3D structures illustrating the dynamics of di-, tri- and tetranucleosome array particles.

From: Angle between DNA linker and nucleosome core particle regulates array compaction revealed by individual-particle cryo-electron tomography

Fig. 3

a, 27 representative cryo-ET density maps from individual particle reconstructions of dinucleosomes in 20 mM HEPES buffer with 5 mM Na+. Each of the density maps is super-imposed with its flexibly fitted model. b, Zoomed-in views of a representative map with its fitting model. DNA color-encoded by their bp index (from yellow to cyan) and histone colored in pink. c, Histograms of the wrapping angle α and the bending angles β. d, Histogram of the core-to-core distance measured between the i and i + 1 NCP. e-h The structure and dynamics of trinucleosomes under same incubation conditions. i–l The structure and dynamics of tetranucleosome under same incubation conditions. m–p The structure and analysis of the same tetranucleosome under higher salt condition (50 mM Na+) after the same incubation time. The red arrow indicates the major change of the peaks.

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