Fig. 5: Simulating chromatin morphology and conformational regulation mechanism through linker DNA angles. | Nature Communications

Fig. 5: Simulating chromatin morphology and conformational regulation mechanism through linker DNA angles.

From: Angle between DNA linker and nucleosome core particle regulates array compaction revealed by individual-particle cryo-electron tomography

Fig. 5

a, In silico assembly of a chromatin fiber by connecting 100 NCPs (top panel) using the α, β angle distribution (top right panel) and the NCP unwrapping distribution derived from experimental statistics of tetranucleosome arrays in 5 mM Na+. Zoomed-in view of the spatial orientation of a representative NCP (in cyan) with its linker DNA (in yellow). b, Ten additional simulated chromatin fiber (100 NCPs) generated from the same distribution of experimental data. c, In silico assembly of a chromatin fiber (connecting 100 NCPs) based on the experimental distributions of angles and the NCP unwrapping levels measured from the tetranucleosome sample with 50 mM Na+ after 20-min incubation. Zoomed-in view of the NCP (in cyan) with its linker DNA (in yellow). d, Ten more chromatin fibers generated based on the same distribution.

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