Fig. 1: Endogenous IL-22RA1 signaling regulates oxidative homeostasis and insulin quality control in human islets. | Nature Communications

Fig. 1: Endogenous IL-22RA1 signaling regulates oxidative homeostasis and insulin quality control in human islets.

From: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

Fig. 1

a mRNA fold change of IL-22RA1 gene expression in healthy lean versus T2D human donor islets, relative to control GAPDH. b Total insulin, c proinsulin secretion (ng/10 islets/30 min), and d proinsulin: insulin ratio from human donor islets during glucose stimulated insulin secretion following treatment 10 µg mL−1 anti-IL22RA1 (p = 0.0341). e mRNA fold change of spliced XBP-1 (sXBP-1; p = 0.0225) and f NOS2 in human donor islets, relative to control GAPDH following treatment with 10 µg mL−1 anti-IL22RA1. g Intracellular nitrite production (µM) in human donor islets following 24 h treatment with 10 µg mL−1 anti-IL22RA1. All graphs presented as Mean ± SEM. a n = 3 biologically independent donors, Two-tailed Mann-Whitney Test; b–g n = 3 biologically independent human islet donors, Kruskal-Wallis test with Dunn’s multiple comparisons test. *p < 0.05; n.s., non-significant. *versus vehicle (anti-IgG) control. Source data are provided as a Source Data file.

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