Fig. 3: Endogenous pancreatic β-cell IL-22ra1 signaling is a key regulator of insulin biosynthesis.
a Pancreatic sections stained for insulin and proinsulin. b Mean intensity per islet area of insulin and proinsulin. c Total serum insulin (p = 0.0175) and proinsulin (p = 0.0452) in animals at 20 weeks of age. d Serum proinsulin: insulin ratio (e) Heatmap showing mRNA fold change of insulin secretion and (f) insulin signaling markers, in whole pancreatic tissue, relative to control housekeeping gene Ywhaz. Box plots in (b–d) display the median (central line), 25th to 75th percentile (box) and minimum to maximum values (whiskers). Female animals; (a–b) n = 12 independent islets from 3 biologically independent animals (insulin), n = 15 independent islets from 3 biologically independent animals (WT, proinsulin), n = 12 independent islets from 3 biologically independent animals (IL-22ra1ΔβKO, proinsulin). c, d n = 8 biologically independent wildtype (IL-22rafl/fl) and 9 biologically independent IL-22ra1ΔβKO animals; e, f n = 7 biologically independent animals. Two-tailed unpaired Student’s t-test; *p < 0.05, **p < 0.01; n.s., non-significant. *versus wildtype (IL-22rafl/fl) littermates. Scale bar: 20 um. Source data are provided as a Source Data file.
