Fig. 4: Endogenous pancreatic β-cell IL-22ra1 signaling modulates islet growth and regeneration.
a H&E sections from pancreatic tissue. b Pancreatic islet area (p < 0.0001), and c frequency distribution of islet areas by size from serial pancreatic sections in animals at 20 weeks of age. d Absolute islet counts in animals following pancreatic islet isolation at 20 weeks of age (p < 0.0001). e Heatmap showing mRNA fold change of islet growth/regeneration markers in whole pancreatic tissue, relative to control housekeeping gene Ywhaz. f Immunohistochemical staining of pancreatic sections for Ki-67 at 8 weeks of age. g Number of Ki-67+ cells per islet area (p = 0.0090). All graphs are presented as Mean ± SEM. Female animals; a–c n = 165 independent islets (all islets in serial sections) from 3 biologically independent wildtype (IL-22rafl/fl), and 115 independent islets (all islets in serial sections) from 3 biologically independent IL-22ra1ΔβKO animals. d n = 3 biologically independent wildtype (IL-22rafl/fl) and 4 biologically independent IL-22ra1ΔβKO animals. f, g n = 16 independent islets (all islets in one section) from 3 biologically independent wildtype (IL-22rafl/fl), and 10 independent islets (all islets in one section) from 3 biologically independent IL-22ra1ΔβKO animals. e n = 7 biologically independent animals; Two-tailed unpaired Student’s t-test. **p < 0.01, ****p < 0.0001; n.s., non-significant. * versus wildtype (IL-22rafl/fl) littermates. Scale Bar: 50 um. Source data are provided as a Source Data file.
