Fig. 5: Lack of endogenous pancreatic β-cell IL-22ra1 signaling causes islet dysfunction due to increased inflammation and cellular stress.
a Heatmap showing mRNA fold change in cellular stress markers and, b inflammatory markers in whole pancreatic tissue, relative to control housekeeping gene Ywhaz. c Mean intensity of Grp-78 per islet area (p = 0.0386) and representative image. d Mean intensity of 4-Hne per islet area (p = 0.0252) and representative image. e Percentage of islet area stained by Iba-1 (p = 0.0024) and representative image. f Percentage of islet area stained by MHC II (p = 0.0034) and representative image. All bar graphs are presented as Mean ± SEM. Female animals; a, b n = 7 biologically independent animals. c n = 15 independent islets (all islets in one section) from 3 biologically independent wildtype (IL-22rafl/fl), and 12 independent islets (all islets in one section) from 3 biologically independent IL-22ra1ΔβKO animals. d n = 7 independent islets (all islets in one section) from 3 biologically independent wildtype (IL-22rafl/fl), and 6 independent islets (all islets in one section) from 3 biologically independent IL-22ra1ΔβKO animals. e n = 13 independent islets (all islets in one section) from 3 biologically independent wildtype (IL-22rafl/fl), and 10 independent islets (all islets in one section) from 3 biologically independent IL-22ra1ΔβKO animals. f n = 14 independent islets (all islets in one section) from 3 biologically independent wildtype (IL-22rafl/fl), and 10 independent islets (all islets in one section) from 3 biologically independent IL-22ra1ΔβKO animals. Two-tailed unpaired Student’s t-test. *p < 0.05, **p < 0.01; n.s., non-significant. *versus wildtype (IL-22ra1fl/fl) littermates. Scale bar: 20 um. Source data are provided as a Source Data file.
