Fig. 6: Ablation of endogenous pancreatic β-cell IL-22ra1 signaling leads to poor quality insulin secretion and reduced glucose uptake. | Nature Communications

Fig. 6: Ablation of endogenous pancreatic β-cell IL-22ra1 signaling leads to poor quality insulin secretion and reduced glucose uptake.

From: Pancreatic beta-cell IL-22 receptor deficiency induces age-dependent dysregulation of insulin biosynthesis and systemic glucose homeostasis

Fig. 6

a Total insulin secretion (ng/ug protein/min) (b) proinsulin secretion (ng/ug protein/min) (90 min; p = 0.0459), and c proinsulin: insulin ratio of mouse islets during in-vitro glucose stimulated insulin secretion, following stimulation with 2.8 mM glucose, 20 mM glucose and 20 mM glucose + 100 nM GLP-1. (60 min; p = 0.0182, 90 min; p = 0.0056, AUC; p = 0.0076). d 2-NBDG uptake in 3T3-L1 adipocytes exposed to 2 ng/mL islet insulin secretion following stimulation with 20 mM glucose + 100 nM GLP-1, p = 0.0054. All graphs in (a-c) are presented as Mean ± SEM, box plots in (d) display the median (central line), 25th to 75th percentile (box) and minimum to maximum values (whiskers). Female animals; a–c n = 13 independent samples (10 islets/sample) from 3 biologically independent wildtype (IL-22rafl/fl), and 19 independent samples (10 islets/sample) from 4 biologically independent IL-22ra1ΔβKO animals. RM two-way ANOVA with the Geisser-Greenhouse correction and Sidak’s multiple comparisons test (line graphs); two-tailed Mann-Whitney Test (bar/box plots). d n = 8 (IL-22rafl/fl) and 9 (IL-22ra1ΔβKO) independent samples. *p < 0.05; n.s., non-significant; **p < 0.01; n.s., non-significant. *versus wildtype (IL-22rafl/fl) control. Source data are provided as a Source Data file.

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