Fig. 3: Metabolic N2O production mechanism.
From: Ammonium-derived nitrous oxide is a global source in streams
a Phylogenetic diagram of the genome associated with metagenomic recombinant binning. Based on the 16S rRNA sequence, phylogenetic relationships were calculated using the maximum likelihood method. The sequences associated with the N cycle based on the NCBI database and metagenome-assembled genomes (MAGs) identified in this study are shown in black and red, respectively. Each MAG was labeled as bin.“MAG source”.“MAG number” and included River (Bai River, B; Juma River, J; Cao River, C; Tang River, T and Zhulong River, Z), Site (1, 2, 3, 4, and 5), Zone (Riparian zone, R; Riverbed zone, O), and Season (Summer, S; Winter, W). The species, abundance, and metabolic path diagrams of nitrifiers and denitrifiers are shown above and below the orange dotted line. b Relative abundance of 15 MAGs and gene recombination and characterization of N2O production- and metabolism-related microbial processes. The cyan bar graph represents the relative abundance (TPM) of 15 MAGs. Different colors in the grid represent different gene abundances (TPMs). c Metabolic relationship of substances related to N2O production. The bin.T2B.7 genome and the bin.B5Z.4 genome represent nitrifiers and denitrifiers, respectively. The black arrows in the figure indicate material transfer, transformation, or electron transport. The yellow, green, brown, and pink arrows represent the NN, ND, NCD, and HD pathways, respectively, in the nitrogen cycle. Filled blocks of different shapes or colors represent different enzymes in cells.
