Fig. 1: CD5L⁻ mice have a compromised immune response to mid-grade CLP.

WT and CD5L⁻ mice were subjected to cecal ligation and puncture (CLP) surgery to induce moderate disease severity. a Kaplan–Meier survival curves were generated to compare survival between the two groups and significance was determined by log-rank (Mantel-Cox) test. Graphical representation of pooled individuals (n = 15 in each group) from 5 independent experiments. b Quantification of body weight loss in the experimental setting described in a. Data are presented as mean values ± SEM. c–e WT and CD5L⁻ mice were subjected to mid-grade CLP; control mice (sham) underwent the same surgical procedure but without ligation and puncture of the cecum. Mice were sacrificed 6, 24, or 72 h after surgery. Pooled data from at least 2 independent experiments. c CFU counts of the indicated tissues from WT and CD5L⁻ mice after mid-grade CLP. Statistical differences between groups were analyzed by two-tailed Mann-Whitney test. DL, detection limit = 20 CFU. Mice per group at 6, 24, and 72 h: Peritoneum WT: 7, 6, and 7; peritoneum CD5L⁻: 6, 6, and 8. Blood WT: 7, 6, and 7; blood CD5L⁻: 8, 6, and 8. Lung, liver, and kidney WT: 7, 7, and 7; lung, liver and kidney CD5L⁻: 7, 6, and 8. d Absolute number of total CD45⁺ leukocytes, CD45⁺CD11b⁺Ly6G⁺ neutrophils, CD45⁺CD11b⁺CD11c^-F4/80⁺ macrophages and Ly6C⁺ and CD206⁺ subsets within macrophage population, assessed by flow cytometry of cell populations in the peritoneal cavity. Statistical comparisons were drawn after performing two-tailed unpaired t-tests with Welch’s correction. Mice per group at 6, 24, and 72 h: Sham, WT, and CD5L⁻: 4, 4, and 4. CLP WT: 7, 7, and 7; CLP CD5L⁻: 7, 6, and 8. Floating bars show the minimum, average (line), and maximum values within each group (c-d). e Fold change in the indicated cytokines between CD5L⁻ and WT mice, quantified by bead-based multiplex immunoassay in samples from the peritoneal cavity (left panel) and blood serum (right panel).