Fig. 5: Biodistribution of CD5L upon mid-grade CLP.

a Normalized counts of Cd5l transcripts obtained from RNA-seq analysis of peritoneal cells of WT mice at indicated times after CLP. Mice per group: 3. b RT-qPCR quantification of Cd5l expression, normalized with Hprt1, in peritoneal cells and liver tissue at indicated times after CLP. Mice per group: 3. c ELISA quantification of CD5L in peritoneum and blood of WT mice at indicated times after mid-grade CLP, or in sham operated animals. Mice per group: at 0 h: 8 in peritoneum, 9 in blood; CLP at 6, 24, and 72 h (peritoneum and blood): 7, 9, and 7; Sham at 6, 24, and 72 h (peritoneum and blood): 6, 6, and 4. d CD5L⁻ mice were subjected to mid-grade CLP and 3 h later injected IV with 2.5 mg/kg rCD5L, or PBS. Fluids from peritoneum and serum were collected 1 h later and total CD5L was quantified by ELISA. DL, detection limit: 6.25 pg/ml. Mice per group: 4 for PBS; 5 for rCD5L. Pooled data from at least 2 independent experiments (c, d). e Scatterplot illustrates the correlation between peritoneal and blood rCD5L levels following IV injection, accompanied by Spearman’s rank correlation coefficient and the two-tailed P value (n = 5). Statistical differences between groups analyzed by one-way ANOVA with Dunnett’s multiple comparisons correction (b), two-tailed unpaired t-tests with Welch’s correction (c), or two-tailed Mann-Whitney test (d). Floating bars show the minimum, average, and maximum values within each group.