Fig. 6: CD5L bioactivity hinges on its free state dynamics.

a Determination of IgM-bound and free endogenous CD5L in peritoneal fluid and serum of WT mice at specified time points after CLP. Values were obtained through densitometric analysis of western blots normalized to baseline IgM-bound CD5L levels (100) in naïve mice. Each group comprised six mice. b ELISA quantification of total IgM in the peritoneum and serum of WT mice at specified times after CLP. Mice per group at 0, 6, 24, and 72 h were as follows: Peritoneum: 7, 9, 7, and 7; Blood: 8, 9, 6, and 7, respectively. c ELISA quantification of total CD5L in the peritoneal cavity of mice subjected to high-grade CLP, and IP- or IV-treated with rCD5L. At 6 h, mice had received one dose of rCD5L (2.5 mg/kg), or PBS, 3 h after CLP. At 24 h, mice had received 2 doses of rCD5L, or PBS, 3 and 6 h after CLP. Mice per group: IP treatment: 6 untreated and 6 treated, at 6 h; 7 untreated and 5 treated, at 24 h. IV treatment: 8 untreated and 6 treated, at 6 h; 8 untreated and 6 treated, at 24 h. d Determination of IgM-bound and free total (endogenous + recombinant) CD5L in the peritoneal cavity of WT mice treated with 2.5 mg/kg rCD5L, or PBS (untreated), via IP or IV routes, 3 h after high-grade CLP, and euthanized 3 h later. Values were obtained by densitometric analysis of western blot bands corresponding to IgM-bound and free CD5L. Mice per group: IP treatment: 6 untreated and 5 treated. IV treatment: 5 untreated and 6 treated. Pooled data from at least 2 independent experiments (a–d). Statistical differences between groups analyzed by two-tailed unpaired t-tests with Welch’s correction (a), one-way ANOVA with Dunnett’s multiple comparisons correction (b), or two-tailed Mann-Whitney test (c, d). Floating bars show the minimum, average, and maximum values within each group.