Fig. 2: DA1 acts genetically with WUS to control SAM size.
From: The peptidase DA1 cleaves and destabilizes WUSCHEL to control shoot apical meristem size
a SAMs of Ler (n =  55), da1-1Ler(n =  56), clv1-1(n = 63), da1-1Ler clv1-1(n = 62), clv2-1(n = 47), da1-1Ler clv2-1(n = 58), clv3-2 (n = 63), and da1-1Ler clv3-2(n = 21). b The average SAM area of Ler (n =  55), da1-1Ler(n =  56), clv1-1(n = 63), da1-1Ler clv1-1(n = 62), clv2-1(n = 47), da1-1Ler clv2-1(n = 58), clv3-2 (n = 63), and da1-1Ler clv3-2(n = 21). c SAMs of Ler, da1-1Ler, wus-1 and da1-1Ler wus-1(n = 20). d SAM of Ler, da1-1Ler, wus-7 and da1-1Ler wus-7(n = 46). e The average area of Ler, da1-1Ler, wus-7, and da1-1Ler wus-7(n  =  46) SAMs. f The relative expression levels of a set of genes, which have been reported to be upregulated or downregulated by the inducible activation of WUS. SAMs of 6-day-old Col-0 and da1-1 were used to perform quantitative RT-PCR assay. Data was normalized with ACTIN2. Data are mean ± S.D (n = 3 for three biological repeats) relative to the wild-type value. P values are from two-sided Student’s t tests. **P < 0.01 compared with the wild type (Col-0). Data in b and e are presented as mean values ± s.e.m. One-way ANOVA with Tukey’s multiple comparison test was used for statistical analyses (P < 0.05). Scale bars, 50 μm (a, c, d). All the plants were grown for 6 days in long-day conditions. The experiments in a, c, d were done with similar results in at least two independent replicates. Source data are provided as a Source Data file.
